Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy.
Identifieur interne : 001255 ( Main/Exploration ); précédent : 001254; suivant : 001256Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy.
Auteurs : R H Farahi [États-Unis] ; A M Charrier [France] ; A. Tolbert [États-Unis] ; A L Lereu [États-Unis, France] ; A. Ragauskas [États-Unis] ; B H Davison [États-Unis] ; A. Passian [États-Unis]Source :
- Scientific reports [ 2045-2322 ] ; 2017.
Descripteurs français
- KwdFr :
- Lignine (ultrastructure), Microscopie à force atomique (MeSH), Nanotechnologie (MeSH), Paroi cellulaire (composition chimique), Paroi cellulaire (ultrastructure), Phénomènes biomécaniques (MeSH), Plasticité cellulaire (MeSH), Populus (composition chimique), Populus (cytologie), Populus (ultrastructure), Élasticité (MeSH).
- MESH :
- composition chimique : Paroi cellulaire, Populus.
- cytologie : Populus.
- ultrastructure : Lignine, Microscopie à force atomique, Nanotechnologie, Paroi cellulaire, Phénomènes biomécaniques, Plasticité cellulaire, Populus, Élasticité.
English descriptors
- KwdEn :
- MESH :
- chemical , ultrastructure : Lignin.
- chemistry : Cell Wall, Populus.
- cytology : Populus.
- ultrastructure : Cell Wall, Populus.
- Biomechanical Phenomena, Cell Plasticity, Elasticity, Microscopy, Atomic Force, Nanotechnology.
Abstract
The complex organic polymer, lignin, abundant in plants, prevents the efficient extraction of sugars from the cell walls that is required for large scale biofuel production. Because lignin removal is crucial in overcoming this challenge, the question of how the nanoscale properties of the plant cell ultrastructure correlate with delignification processes is important. Here, we report how distinct molecular domains can be identified and how physical quantities of adhesion energy, elasticity, and plasticity undergo changes, and whether such quantitative observations can be used to characterize delignification. By chemically processing biomass, and employing nanometrology, the various stages of lignin removal are shown to be distinguished through the observed morphochemical and nanomechanical variations. Such spatially resolved correlations between chemistry and nanomechanics during deconstruction not only provide a better understanding of the cell wall architecture but also is vital for devising optimum chemical treatments.
DOI: 10.1038/s41598-017-00234-4
PubMed: 28273953
PubMed Central: PMC5428038
Affiliations:
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Ragauskas</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Davison, B H" sort="Davison, B H" uniqKey="Davison B" first="B H" last="Davison">B H Davison</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Passian, A" sort="Passian, A" uniqKey="Passian A" first="A" last="Passian">A. Passian</name><affiliation wicri:level="1"><nlm:affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Physics, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physics, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28273953</idno><idno type="pmid">28273953</idno><idno type="doi">10.1038/s41598-017-00234-4</idno><idno type="pmc">PMC5428038</idno><idno type="wicri:Area/Main/Corpus">001413</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001413</idno><idno type="wicri:Area/Main/Curation">001413</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001413</idno><idno type="wicri:Area/Main/Exploration">001413</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy.</title><author><name sortKey="Farahi, R H" sort="Farahi, R H" uniqKey="Farahi R" first="R H" last="Farahi">R H Farahi</name><affiliation wicri:level="1"><nlm:affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Charrier, A M" sort="Charrier, A M" uniqKey="Charrier A" first="A M" last="Charrier">A M Charrier</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, CNRS, CINaM, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, CNRS, CINaM, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Tolbert, A" sort="Tolbert, A" uniqKey="Tolbert A" first="A" last="Tolbert">A. Tolbert</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332</wicri:regionArea><wicri:noRegion>30332</wicri:noRegion></affiliation></author><author><name sortKey="Lereu, A L" sort="Lereu, A L" uniqKey="Lereu A" first="A L" last="Lereu">A L Lereu</name><affiliation wicri:level="1"><nlm:affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille Univ, CNRS, CINaM, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille Univ, CNRS, CINaM, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Ragauskas, A" sort="Ragauskas, A" uniqKey="Ragauskas A" first="A" last="Ragauskas">A. Ragauskas</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Davison, B H" sort="Davison, B H" uniqKey="Davison B" first="B H" last="Davison">B H Davison</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Passian, A" sort="Passian, A" uniqKey="Passian A" first="A" last="Passian">A. Passian</name><affiliation wicri:level="1"><nlm:affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Physics, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physics, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomechanical Phenomena (MeSH)</term><term>Cell Plasticity (MeSH)</term><term>Cell Wall (chemistry)</term><term>Cell Wall (ultrastructure)</term><term>Elasticity (MeSH)</term><term>Lignin (ultrastructure)</term><term>Microscopy, Atomic Force (MeSH)</term><term>Nanotechnology (MeSH)</term><term>Populus (chemistry)</term><term>Populus (cytology)</term><term>Populus (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Lignine (ultrastructure)</term><term>Microscopie à force atomique (MeSH)</term><term>Nanotechnologie (MeSH)</term><term>Paroi cellulaire (composition chimique)</term><term>Paroi cellulaire (ultrastructure)</term><term>Phénomènes biomécaniques (MeSH)</term><term>Plasticité cellulaire (MeSH)</term><term>Populus (composition chimique)</term><term>Populus (cytologie)</term><term>Populus (ultrastructure)</term><term>Élasticité (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Paroi cellulaire</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Wall</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomechanical Phenomena</term><term>Cell Plasticity</term><term>Elasticity</term><term>Microscopy, Atomic Force</term><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Lignine</term><term>Microscopie à force atomique</term><term>Nanotechnologie</term><term>Paroi cellulaire</term><term>Phénomènes biomécaniques</term><term>Plasticité cellulaire</term><term>Populus</term><term>Élasticité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The complex organic polymer, lignin, abundant in plants, prevents the efficient extraction of sugars from the cell walls that is required for large scale biofuel production. Because lignin removal is crucial in overcoming this challenge, the question of how the nanoscale properties of the plant cell ultrastructure correlate with delignification processes is important. Here, we report how distinct molecular domains can be identified and how physical quantities of adhesion energy, elasticity, and plasticity undergo changes, and whether such quantitative observations can be used to characterize delignification. By chemically processing biomass, and employing nanometrology, the various stages of lignin removal are shown to be distinguished through the observed morphochemical and nanomechanical variations. Such spatially resolved correlations between chemistry and nanomechanics during deconstruction not only provide a better understanding of the cell wall architecture but also is vital for devising optimum chemical treatments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28273953</PMID><DateCompleted><Year>2018</Year><Month>09</Month><Day>03</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>03</Month><Day>10</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy.</ArticleTitle><Pagination><MedlinePgn>152</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-017-00234-4</ELocationID><Abstract><AbstractText>The complex organic polymer, lignin, abundant in plants, prevents the efficient extraction of sugars from the cell walls that is required for large scale biofuel production. Because lignin removal is crucial in overcoming this challenge, the question of how the nanoscale properties of the plant cell ultrastructure correlate with delignification processes is important. Here, we report how distinct molecular domains can be identified and how physical quantities of adhesion energy, elasticity, and plasticity undergo changes, and whether such quantitative observations can be used to characterize delignification. By chemically processing biomass, and employing nanometrology, the various stages of lignin removal are shown to be distinguished through the observed morphochemical and nanomechanical variations. Such spatially resolved correlations between chemistry and nanomechanics during deconstruction not only provide a better understanding of the cell wall architecture but also is vital for devising optimum chemical treatments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Farahi</LastName><ForeName>R H</ForeName><Initials>RH</Initials><AffiliationInfo><Affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Charrier</LastName><ForeName>A M</ForeName><Initials>AM</Initials><Identifier Source="ORCID">0000-0002-0205-1341</Identifier><AffiliationInfo><Affiliation>Aix Marseille Univ, CNRS, CINaM, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tolbert</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, 30332, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lereu</LastName><ForeName>A L</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Aix Marseille Univ, CNRS, CINaM, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davison</LastName><ForeName>B H</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Passian</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Quantum Information Science, Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. passianan@ornl.gov.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Physics, University of Tennessee, Knoxville, TN, 37996, USA. passianan@ornl.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>03</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001696" MajorTopicYN="N">Biomechanical Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000066670" MajorTopicYN="N">Cell Plasticity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004548" MajorTopicYN="N">Elasticity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018625" MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036103" MajorTopicYN="N">Nanotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>08</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>02</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28273953</ArticleId><ArticleId IdType="doi">10.1038/s41598-017-00234-4</ArticleId><ArticleId IdType="pii">10.1038/s41598-017-00234-4</ArticleId><ArticleId IdType="pmc">PMC5428038</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Chem Biol. 2010 Oct;6(10):724-32</Citation><ArticleIdList><ArticleId 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IdType="pubmed">23180856</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Farahi, R H" sort="Farahi, R H" uniqKey="Farahi R" first="R H" last="Farahi">R H Farahi</name></noRegion><name sortKey="Davison, B H" sort="Davison, B H" uniqKey="Davison B" first="B H" last="Davison">B H Davison</name><name sortKey="Davison, B H" sort="Davison, B H" uniqKey="Davison B" first="B H" last="Davison">B H Davison</name><name sortKey="Farahi, R H" sort="Farahi, R H" uniqKey="Farahi R" first="R H" last="Farahi">R H Farahi</name><name sortKey="Farahi, R H" sort="Farahi, R H" uniqKey="Farahi R" first="R H" last="Farahi">R H Farahi</name><name sortKey="Lereu, A L" sort="Lereu, A L" uniqKey="Lereu A" first="A L" last="Lereu">A L Lereu</name><name 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Passian</name><name sortKey="Passian, A" sort="Passian, A" uniqKey="Passian A" first="A" last="Passian">A. Passian</name><name sortKey="Passian, A" sort="Passian, A" uniqKey="Passian A" first="A" last="Passian">A. Passian</name><name sortKey="Passian, A" sort="Passian, A" uniqKey="Passian A" first="A" last="Passian">A. Passian</name><name sortKey="Ragauskas, A" sort="Ragauskas, A" uniqKey="Ragauskas A" first="A" last="Ragauskas">A. Ragauskas</name><name sortKey="Ragauskas, A" sort="Ragauskas, A" uniqKey="Ragauskas A" first="A" last="Ragauskas">A. Ragauskas</name><name sortKey="Tolbert, A" sort="Tolbert, A" uniqKey="Tolbert A" first="A" last="Tolbert">A. Tolbert</name><name sortKey="Tolbert, A" sort="Tolbert, A" uniqKey="Tolbert A" first="A" last="Tolbert">A. Tolbert</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Charrier, A M" sort="Charrier, A M" uniqKey="Charrier A" first="A M" last="Charrier">A M Charrier</name></region><name sortKey="Lereu, A L" sort="Lereu, A L" uniqKey="Lereu A" first="A L" last="Lereu">A L Lereu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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